Heparins are glycosaminoglycans, which are used for the inhibition of blood coagulation. After the isolation out of animal products it has to guaranteed that the contaminant OSCS (oversulfated chondroitin sulfate) is not included. As heparin has no aromatic structure the detection is made at low wave length. To minimize the interaction of the molecule with the capillary wall a highly concentrated electrolyte is used.
Heparin and OSCS
- Separation: CZE
- Electrolyte: 850 mM Tris-phosphate
- Capillary: fused silica, 20 µm ID, 64 cm in total
- Detection: direct UV, 200 nm
- Description: The USP SST solution contains heparin and OSCS (oversulfated chondrointin sulfate) to ensure the saparation.
Heparin SST
Download: Heparin_SST.pdf
Heparin sample solution
- Separation: CZE
- Electrolyte: 850 mM Tris-phosphate
- Capillary: fused silica, 20 µm ID, 64 cm in total
- Detection: direct UV, 200 nm
- Description: The sample solution ic checked for its purity regarding OSCS (oversulfated chondrointin sulfate). The shown sample fulfills the specification.
Heparin sample solution
Download: Heparin_Probeloesung.pdf