Miscellaneous

Frequently samples comprise of mixtures of analytes which cannot be assigned to a specific category. Therefore „miscellaneous“ is a collection of exceptional applications.

Urea

Urea (other names: Carbamid or E927b) is a natural metabolic product of mammals and is an important part of the nitrogen circle. On an industrial scale urea is produced from ammoniak.urea-formelUrea is highly soluble in water. The analysis of urea with capillary electrophoresis is possible due to its affinity to micelles. Allthough it iss mall, it is sufficient to separate urea from the EOF. Detection takes place at low wave lengths with direct UV detection.

  • Separation: MEKC
  • Electrolyte: Borate/SDS
  • Capillary: fused silica, 50 µm ID, bubble cell, 64 cm in total
  • Injection: 10 s, 50 mbar
  • Detection: direct UV, 200 nm
  • Description: The linearity for the quantitation of urea was tested in the range of 20 mg/l up to 1000 mg/l.
analysis of urea Harnstoff, CE

Urea analysis: linearity

Download:  urea-linearity.pdf

  • Description: The amount of urea was determined in a body lotion and a pharmaceutical ointment for dry skins as described in the method above. The samples were extracted with pure water and injected instantly.
analysis of urea Harnstoff, CE

Urea in a  body lotion

Download: urea1.pdf

  • Description: The hand cream was extracted with pure water and examined for urea.
analysis of urea Harnstoff, CE

Urea in a hand cream

Download: urea2.pdf

Benzamide, o-toluenesulfonic acid (o-TSA) und saccharin

  • Separation: CZE
  • Electrolyte: Borate/SDS
  • Capillary: fused silica, 50 µm ID, bubble cell, 64 cm in total
  • Injection: 20 s, 50 mbar
  • Detection: direct UV, 200 nm
  • Description: The standard solution contains 10 mg/l benzamide, o-toluenesulfonic acid (o-TSA) and saccharin, each. Similar mixtures as used for industrial cleaning.
Sonstiges_1

Cleaning solution with benzamide, toluenesulfonic acid and saccharin

Download: Reinigungslösung.pdf

Nucleotide sugars

  • Mode: CZE
  • Electrolyte: Borate
  • Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm total
  • Injection: hydrodynamic
  • Detection: direct UV, 200 nm
  • Description: Nucleotide sugars can be analyzed using a Borate electrolyte. The PDF shows the separation of the six nucleotide sugars: CMP-sialic (CAS 3063-71-6),UDP-GlcNAc (CAS 91183-98-1), GDP-Man (103301-73-1), GDP-Fuc (15839-70-0), UDP-Glc and UDP-Gal (CAS 137868-52-1). This method can be used for the determination of nucleotide sugars in cells.
Nukleotid Zucker, CE, Nukleotide

Nucleotide sugars

Download: nucleotide-sugars.pdf

Nicotine

  • Mode: MEKC
  • Electrolyte: Borate and SDS
  • Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm total
  • Injection: hydrodynamic
  • Detection: direct UV, 200 nm
  • Description: Nicotine (C10H14N2; CAS: 54-11-5) can be analyzed using an electrolyte containing SDS. The PDF shows the spectral data and the calibration of nicotine.
Nicotine, Nikotin, CE

Nicotine standard solution

Download: nicotine-1.pdf

  • Description: Nicotine (C10H14N2; CAS: 54-11-5) can be analyzed in various matrices. Sample preparation is done only by extraction using ethanol. The PDF shows the determination of nicotine in chewing gum, patches, tobacco and a solution of an E-cigarette.
Nicotine, Nikotin, CE

Nicotine in different samples

Download: nicotine-2.pdf