Sweeteners

For severeal years the household sugar sucrose has been successively substituted by sweeteners to create foods with lower calories and that are anti-caries but nevertheless sweet. The american trend sweet`n`low has reached Europe and leeds to a steady search for new low calorie sweeteners. In the meantime a quantity of different substances is approved in the USA and the EU (see table). Frequently mixtures of sweeteners are used to round off the sweet taste and to simulate the much appreciated taste of sugar. The palette of approved sweeteners ranges from sugar alcohols and synthetic products to herbal sweeteners. The analytical aim is to determine as much compounds as possible simultaneously, which is quite a challenge because oft he diversity of the substances (table publication: Süßstoffe in Lebensmitteln). For the separation a micellar electrolyte is used for all relevent substances and UV detection is done at low wavelength.

Sweeteners standard solution

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The standard solution contains twelve different sweeteners. Due to the instability of aspartame phenylalanine and aspartic acid are visible after 24 h (see PDF page 2).

Download: Suessstoffe_Standard.pdf

Sweeteners in a powdery artificial sweetener

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The sample had a concentration of 1000 mg/l. Cyclamate and saccharin were found.

Download: Suessstoffe_Pulver.pdf

Sweeteners in a liquid artificial sweetener

• • Separation: MEKC
  • Electrolyte: Borate-SDS
  • Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
  • Injection: 10 s, 50 mbar
  • Detection: direct UV, 195 nm
  • Description: The sample had a concentration of 1000 mg/l. Cyclamate and saccharin were found.

Download: Suessstoffe_fluessig.pdf

Sweeteners in a sage drink

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The sample had a concentration of 1000 mg/l. Aspartame and acesulfam were found. As another additive ascorbic acid (vitamin C) was detected.

Download: Suessstoffe_Salbei.pdf

Sweeteners in a chewing gum (brand 1)

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The chewing gum was homogenized in a concentration of 5000 mg/l and subsequently fitrated. Maltitol, aspartame, sorbit, saccharin and acesulfam as well as two unknown substances were detected.

Download: Suessstoffe_Kaugummi_1.pdf

Sweeteners in a chewing gum (brand 2)

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The chewing gum was homogenized in a concentration of 5000 mg/l and subsequently fitrated. Aspartam, Xylitol, sorbit, and mannit were detected.

Download: Suessstoffe_Kaugummi_2.pdf

Sweeteners in stevia sticks

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The sample was analysed in a concentration of 10000 mg/l. Erythrite and the steviol glycoside Rebaudiosid A are included.

Download: Suessstoffe_Stevia_Sticks.pdf

Sweeteners in sugar free candies

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The sample was analysed in a concentration of 2000 mg/l. Xylite was found as sole sweetener as declared.

Download: Suessstoffe_Bonbon.pdf

Sweeteners in eye ointment

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The ointment was analysed in a concentration of 2000 mg/l. Besides saccharin glycerole and the active substance were found.

Download: Suessstoffe_Augensalbe.pdf

Sweeteners in a fitness drink

• Separation: MEKC
• Electrolyte: Borate-SDS
• Capillary: Bubble Cell, fused silica, 50 µm ID, 64 cm in total
• Injection: 10 s, 50 mbar
• Detection: direct UV, 195 nm
• Description: The isotonic fitness drink was tenfold diluted. Besides saccharin fructose as well as an unknown compound were detected.

Download: Suessstoffe_Fitness_Drink.pdf